An interval of "three hours", from 3.34pm to 6.37pm on 19 September 2007, in which the analyzes on the genetic profile found on the pedals of Alberto Stasi's bike went from indicating "a partial profile", not attributable, to providing the match with Chiara Poggi's DNA, then contrasting, among other things, with the two "null runs" of the following day which were never made available to the parties. It is also on this time window that the investigations of the Pavia prosecutors are focused in the new investigations on the Garlasco case, which hypothesize an exchange of test tubes with the DNA of the breakfast spoon. In addition to the closed investigation into Andrea Sempio, which exonerates Stasi to bring the 38-year-old clerk to trial for the crime, there is an open thread that delves into the investigations of 19 years ago.
As can be seen from the questions dated 10 September by Deputy Stefano Civardi to Ris captain Alberto Marino, who was conducting the biological tests, the hypothesis is that the investigators of the time started "from the assumption that Chiara's DNA was on the pedals" and that a series of irregularities returned precisely that data, which led to the arrest of the student (released after 4 days). Shortly after 3pm on September 19th the first tests on the "bu_p" sample returned a "partial trace". The "term 'evaporated'" referring to that profile was noted in the comments of the file. Marino explained that that route, however, "needed" to be "in-depth". So three hours later came what Deputy Civardi calls "the turning point", which still needs to be clarified: the profile of the victim was obtained which "no geneticist" would have questioned again. That data, however, the prosecutors insist, was not uploaded by Marino, like the previous one, but by the operator with the acronym "Port". The captain said, among many "I don't remember", that the "re-amplification" of the sample, to "improve" it, presumably was done by him, while "the loading at this point I assume by Portera", also a former Ris. Precisely in this phase, according to the investigators, there may have been a "sampling exchange" with the DNA from Chiara's breakfast spoon, which recorded the "same quantification in hundredths of nanograms per microlitres". Civardi pointed out in the report: "It's the same date. We tried to understand how it was possible (...) we add the other doubt that once you upload it nothing comes out. Well, in short... Following the scientific method of doubt, perhaps some doubts arise".
The investigators also insisted on another point: to whom and when did Marino communicate the profile match. "There is a document (...) which if I'm not mistaken is the first communication we make to Dr. Muscio", said Marino. "That one is from 5 days later, it's the 24th!", replied Civardi, referring to the now known relationship. In the middle, the two null results never ended up in the records. Marino, when pressed, said he had communicated the positive result on the 19th presumably to the then commander Luciano Garofano. And he specified that he was indeed a consultant to prosecutor Muscio, but was carrying out activities within the scope of the investigation delegation to the Ris. The role of Garofano, who was also heard as a witness, is also being examined, given that he spent a lot personally in the investigations. In an email from February 2009, for example, when Stasi's abbreviated trial began, he pointed out to his team how "counter-deductions" on the topics of the bloodstains, the famous walk and the shoes needed to be improved. “They had already proven themselves clean,” he wrote of the shoes. And he added: "one shoe is enough for us and in any case we are not required to be too specific".
